Shimadzu Nexera X2  

Maximizing the Potential of UHPLC/HPLC Analyses Ultra High Performance Liquid Chromatograph Nexera X2

Intelligence

Intelligent data solutions, including a new  i-PDeA peak calculation method for enhanced separation power, accelerate workflows.

Sensitivity

The ultimate platform for high-sensitivity UHPLC and HPLC detection.

Resolution

Enhanced peak resolution achieved by minimizing external column dispersion.

Stability

Superior stability for UHPLC/HPLC analyses.
Shimadzu Nexera X2 Utilizing patented technologies, and driven by customer input, Shimadzu has developed the Nexera X2 series to deliver superior, real-world performance across a wider application range, while delivering unparalleled flexibility and reliability in UHPLC/HPLC analyses. With no limi

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Shimadzu Nexera XR  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

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Shimadzu Prominence nano  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

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Agilent 1290 Infinity II Refractive Index LC Detector  

The 1290 Infinity II Refractive Index Detector is equipped with an ultralow dispersion microflow cell, which significantly reduces run times for higher sample throughput and improved resolution. Lower solvent consumption means much lower cost of analysis. A high-performance detector of choice for accurate, reproducible, routine analysis of polymers and other compounds that aren’t detectable by UV.
Features Of Agilent 1290 Infinity II Refractive Index LC Detector:
Shorter run times – for considerably higher sample throughput.
Ultralow dispersion – for improved sample definition and resolution.
Reduced solvent consumption – for significant savings in analysis cost.
Ideal tool for polymer analysis – consistent molecular weights, micro or analytical scale.
Excellent sensitiv

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Shimadzu Refractive Index LC Detector 20A (RID-20A)  

Inheriting the stability and extensibility that are the strengths of the Prominence series, the new Shimadzu Refractive Index LC Detector 20A (RID-20A) model of differential refractive index detector is designed with a new reference-cell auto-purge feature and validation support function.

Specifications :

 
Pressure Relief Valve :
The RID-20A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.

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Shimadzu UV-VIS SPD-20A/20AV LC Detectors  

Offering dual-wavelength mode
The SPD-20A/20AV is a UV-VIS detector that takes sensitivity to the limit (Noise level 0.5 x 10(-5) AU), and offers wide linearity (2.5AU). SPD-20A: Wavelength range: 190 to 700nm The SPD-20AV has a mode that allows the deuterium lamp and tungsten lamp to be lit simultaneously, enabling high-sensitivity wavelength-programming detection fro ultraviolet light and the entire visible-light range. Wavelength range 190 to 900nm.

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Shimadzu Photodiode Array SPD-M30A LC Detector  

Supports diverse applications from HPLC to UHPLC
The SPD-M30A detector achieves a 0.4×10-5 AU noise level. The SR-Cell (Sensitivity and Resolution Cell) significantly cuts peak dispersion. This model supports analysis from conventional LC to ultra-fast and UHPLC analysis. The optional high-sensitivity cell has an 85 mm optical path length and is able to detect trace components that were conventionally difficult to detect. The TC-Optics function further improves baseline stability.

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Shimadzu RF-20A/RF-20Axs Fluorescence LC Detector  

The RF-20A/20AXS fluorescence detectors offer world-leading sensitivity and ease-of-maintenance. The RF-20AXS is a high-sensitivity model that incorporates a temperature-controlled cell with a cooling function as standard.

RF-20A / Standard model:
The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at least 1200, as well as excellent ease-of-use with such features as maintenance from the front panel and adoption of a long-life lamp.

RF-20AXS / Achieves World-Leading Sensitivity :
Offering world-class levels of sensitivity and easy maintenance, the RF-20AXS features a water Raman S/N ratio of at least 2000 and a temperature-controlled cell with a cooling function. This maintains a constant detector cell temperature, even if the room temperature

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Shimadzu Photodiode Array SPD-M20A LC Detector  

General-purpose model
The SPD-M20A is a general-purpose model incorporating a deuterium lamp. The light-source compensation function achieves a noise level of 0.6×10-5AU. Cell temperature control ensures baseline stability.
Key Benefits:
Noise level of 0.6×10-5 AU
Superior Linearity - 2.0AU
Ethernet interface & web control functions
Can be incorporated into non-Shimadzu HPLC systems
Light source: Deuterium (D2) lamp, tungsten (W) lamp
Wavelength range: 190 to 800 nm
Wavelength accuracy: 1 nm max.
Wavelength precision: 0.1 nm max
Cell temperature control range: 5°C above room temperatre to 50°C
Operating temperature 4°C to 35°C
Power requirements: 100 VAC, 150 VA, 50/60 Hz
The SPD-M20A PDA is the third member of the UV-Vis absorbance detectors. The M20A, like

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Shimadzu CDD-10AVP Conductivity LC Detector  

Handles a Wide Variety of Analysis Options
The CDD-10AVP conductivity detector achieves an even higher level of sensitivity and makes it possible to perform a wide variety of analysis scenarios with a single unit. An option card enables the simultaneous 2-channel measurement of anions and cations, and a suppressor option allows expansion to a suppressor system for ultra-high sensitivity work. Organic acids can be analyzed using Shimadzu's unique post-column pH-buffered electroconductivity method.
Low noise, low drift and wide dynamic range assure proven performance of the Shimadzu VP series HPLC. Data acquired by the CDD-10AVP is transferred to the SCL system controller via an optical fiber interface and are digitally processed by workstation. By adding the optional cell (dual kit NS

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Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

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P

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Preventing Leaks In HPLC Analyses  

Leaks are a common problem in HPLC analyses. To minimize leaks in your system, avoid interchanging hardware and fittings from different manufacturers. Incompatible fittings can be forced to fit initially, but the separation may show problems and repeated connections may eventually cause the fitting to leak. If interchanging is absolutely necessary, use appropriate adapters and check all connections for leaks before proceeding. Highly concentrated salts (>0.2 M) and caustic mobile phases can reduce pump seal efficiency. The lifetime of injector rotor seals also depends on mobile phase conditions, particularly operation at high pH. In some cases, prolonged use of ion pair reagents has a lubricating effect on pump pistons that may produce small leaks at the seal. Some seals do not perform

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clogged column frit HPLC problem  

Leaks are a common problem in HPLC analyses. To minimize leaks in your system, avoid interchanging hardware and fittings from different manufacturers. Incompatible fittings can be forced to fit initially, but the separation may show problems and repeated connections may eventually cause the fitting to leak. If interchanging is absolutely necessary, use appropriate adapters and check all connections for leaks before proceeding. Highly concentrated salts (>0.2 M) and caustic mobile phases can reduce pump seal efficiency. The lifetime of injector rotor seals also depends on mobile phase conditions, particularly operation at high pH. In some cases, prolonged use of ion pair reagents has a lubricating effect on pump pistons that may produce small leaks at the seal. Some seals do not perform

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1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

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Agilent Technologies 1100 Series

hplcagilent1100.hplchplc.com/

Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System. 1100 hplc,agilent hplc,hplc system,used hplc,used agilent hplc. HPLC System with ...

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Used Agilent 1100 S

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Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

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Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

hplcagilent1260.hplchplc.com/

 

agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1260 HPLC , Agilent 1260 LCSystem,Used Agilent 1260, Infinity Series, HPLC System,DAD ...

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agilent 1260 hplc

watershplc.hplchplc.com/list/agilent+1260+hplc.html

 

This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

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Notes on Cleaning bound Protein from RP HPLC columns  

First, a few comments:

·         Before proceeding with any column regeneration or cleaning procedures, always refer to the specific advice provided by the column manufacturer. Approved maintenance and cleaning instructions can often be found in the product guide which comes with the new column. Their guidelines supersede these!

·         Columns are consumable items. After a suitable amount of use, the time and materials required to regenerate them may cost more than the purchase of a replacement column. Always have a new, spare column on hand.

·         Protect your detector. Before washing or cleaning the column, disconnect the column outlet line and direct the column to waste only.

For RP supports, if buffers have been used, always start by washi

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features :
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resolution gainin fast LC -at up to 120 Hz data acquisition r

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Agilent Technologies is an American public research  

Agilent Technologies is an American public research, development and manufacturing company established in 1999 as a spin-off from Hewlett-Packard. The resulting IPO of Agilent stock was the largest in the history of Silicon Valley at the time.
The company provides analytical instruments, software, services and consumables for the entire laboratory workflow. Agilent focuses its products and services on six markets: food, environmental and forensics, pharmaceutical, diagnostics, chemical and energy, and research. From 1999 to 2014 the company also produced test and measurement equipment, this division was spun out to form Keysight.
Based on 2003 information, Agilent maintained four locations in the San Francisco Bay area: San Jose, Santa Clara, Santa Rosa and Rohnert Park.Santa Clara is an

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features Of Agilent 1260 Infinity II Variable Wavelength Detector:
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resoluti

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Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If

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Troubleshooting HPLC- Loss in Response for all Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If t

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